The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline
Acta cir. bras
; 32(11): 935-948, Nov. 2017. graf
Article
in En
| LILACS
| ID: biblio-886187
Responsible library:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury.Methods:
Thirty male rats were assigned to 5 groups (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX) PTX + ischemia (30 minutes) + reperfusion (60 minutes); (IPC-IR-SS) 5 minutes of ischemia + 5 minutes of reperfusion (IPC) + saline + I(30 minutes)+R(60 minutes); and (IPC-IR-PTX) IPC + PTX + I(30 minutes)+ R(60 minutes).Results:
The application of IPC and PTX showed a significantly lower immunohistochemistry reaction for active caspase-3 (P<0.05) compared to IR+SS. The number of cells immunoreactive to BCL-2 was higher in the IR-PTX group (P>0.05). The NOS-2 expression (qRTPCR) in the IR-PTX group (P<0.05) was higher than the values for the IPC+IR-SS and IPC-IR-PTX groups. The NOS-3 expression was significantly upper in the IPC-IR-PTX group than in the CG (P<0.05), the IR-SS (P<0.05) and the IR-PTX (P<0.05) groups.Conclusions:
The BCL-2 and active caspase-3 showed beneficial effects on PTX and IPC. The expression of NOS-2 and NOS-3 in the IPC and IPC-PTX groups showed no synergistic effect.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Pentoxifylline
/
Apoptosis
/
Nitric Oxide Synthase
/
Ischemic Preconditioning
/
Intestinal Diseases
/
Intestines
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2017
Document type:
Article
Affiliation country: